Human Anti-IL13 Recombinant Antibody (clone CNTO607) (CAT#: PABW-170)
Recombinant Human Antibody (CNTO607) is capable of binding to IL13, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IL13 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- IL13 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. CNTO607 binds human IL-13 with high affinity (Kd=15 pM) and prevents binding of IL-13 to both IL-13Rα1 and IL- 13Rα2. CNTO607 potently neutralizes the activity of IL-13 in a variety of bioassays, including cell survival, eotaxin production, and STAT6 signaling.
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ELISA
Figure 1 Anti-IL13 Recombinant Antibody (PABW-170) in ELISA
ELISA analysis of PABW-170 was performed by coating with human IL13 protein (His tag). Then blocked with BSA and incubated with PABW-170. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-IL13 Recombinant Antibody (PABW-170) in WB
Western blot analysis of PABW-170 was performed by loading human IL13 protein (Lane 1 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABW-170 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-IL13 Recombinant Antibody (PABW-170) in DB
Dot Blot analysis of PABW-170 was performed by coating with human IL13 protein (His tag).
PABW-170 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
SDS-PAGE
Figure 4 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SDS-PAGE
SDS-PAGE analysis of PABW-170 in non-reduced and reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 5 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SEC-HPLC
SEC-HPLC analysis of PABW-170 shows that the purity is more than 95%.
Specifications
- Immunogen
- Human interleukin 13
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human IL13
- Species Reactivity
- Human
- Clone
- CNTO607
- Applications
- ELISA, WB, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Asthma
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-218 | Anti-Human IL13 Recombinant Antibody (Tralokinumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG4 - lambda |
| TAB-068ZJ | Anti-Human IL-13 Recombinant Antibody (01471/G6) | WB, ELISA, Neut | Human antibody |
| TAB-069ZJ | Human Anti-IL13 Recombinant Antibody (TAB-069ZJ) | ELISA, Neut, FC | Human IgG |
| TAB-070ZJ | Human Anti-IL13 Recombinant Antibody (TAB-070ZJ) | ELISA, Neut, FC | Human IgG |
| TAB-067ZJ-F(E) | Anti-Human IL-13 Recombinant Antibody Fab Fragment (CNTO 607) | ELISA, Inhib | Human antibody |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABW-170, RRID: AB_3111690)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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